Primer Design: Melting Temperature and Primer Length

Selective 2' Hydroxyl Acylation Analyzed By Primer Extension (SHAPE)

Brief Intro to Ion Exchange Chromatography

RNA Secondary Structure Motifs & Beyond

New Rapid Amplification of cDNA Ends (5' RACE)

Classic Rapid Amplification of cDNA Ends (3', 5' RACE)

Telomerase Repeat Amplification Protocol (TRAP)

Section 1: Instructional Audio

DNA topology, Branched DNA junctions, Reciprocal Exchange, DNA Knots, DNA Half-Turns & Nodes/Crossings, DNA Helical Conformations (A-DNA, B-DNA, Z-DNA), Switchback-based Trefoil Knot.

Section 5: instructional audio

Topics: Introduction to Telomerase, Telomerase Holoenzyme Purification, More on Telomerase RNA, Telomerase & Cancer